AbeI, a restriction endonuclease from Azotobacter beijerinckii, which recognizes the asymmetric heptanucleotide sequence 5'-CCTCAGC-3'(-5/-2)
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SimI, a new restriction endonuclease that recognizes non-palindromic sequence 5'-GGGTC-3'(-3/0).
Siml, a type II restriction endonuclease, has been isolated from Staphylococcus iniermedius 6H using heparin and hydrooxyapatite chromatographic steps. The crude extract contained -3000 U Siml per gram of cells. Three cleavage positions of Siml on pUC19 DNA (-999, 1480 and 1768) have been mapped by double digests with enzymes Bgll, /tccl 131 (isoschizomer of Seal), Sail, Pvul, NmGl (isoschizome...
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fl«BSI, a type II restriction endonuclease, has been isolated from Bacillus stearothermophilus BS. ZforBSI, an isoschizomer of Sna\ (1), recognizes the six base palindromic sequence and cleaves it as indicated by arrows: 5'GTA1TAC3' 3'CATtATG5'. BsfBSI recognizes three sites on X DNA (Figure 1) . Double digestion mapping with BstBSl and EcoRl, Smal, Kpnl, Mlul and PvuU showed that &/BSI sites w...
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Type II restriction endonucleases recognize and cleave near specific DNA sequences, usually 4-6 basepair palindromes. They are of fundamental importance as tools for the recombinant DNA technology as they provide the selective cleavages required for the analysis and restructuring of DNA in vitro. The flexibility of these techniques is proportional to the number of DNA sequences which can be cle...
متن کاملRestriction endonuclease MvaI is a monomer that recognizes its target sequence asymmetrically
Restriction endonuclease MvaI recognizes the sequence CC/WGG (W stands for A or T, '/' designates the cleavage site) and generates products with single nucleotide 5'-overhangs. The enzyme has been noted for its tolerance towards DNA modifications. Here, we report a biochemical characterization and crystal structures of MvaI in an apo-form and in a complex with target DNA at 1.5 A resolution. Ou...
متن کاملThe restriction endonuclease R.NmeDI from Neisseria meningitidis that recognizes a palindromic sequence and cuts the DNA on both sides of the recognition sequence
The restriction endonuclease Type II R.NmeDI from Neisseria meningitidis 2120 (serogroup C, ST-11 complex) was characterized. The cloned nmeDIR gene was expressed in Escherichia coli cells, and the endonucleolytic and restriction activities of R.NmeDI were then observed in vitro and in vivo. The nmeDIR gene consists of 1056 bp coding 351 aa protein with a calculated molecular weight of M((r)) =...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1998
ISSN: 1362-4962
DOI: 10.1093/nar/26.21.4917